Malaysia has been the most outstanding country for its profitable development. Meanwhile, China is the largest trade partner of Malaysia (Statics Bureau of Malaysia). Therefore, this research employed qualitative study is to analyze and discuss between China and Malaysia cultural differences in business relations by compared with cultural difference in business strategy, decision making, business negotiation and conflict management. By studying cultural differences between China and Malaysia, the results show that knowing another culture will be fundamentally an allowable concern of business activities. Moreover, it is indispensable for those who make the effort to understand another culture and gain knowledge about how to behave in that cultural condition. However, this finding also has a tendency to provide relevant information towards global managers who plan to do international business between China and Malaysia.