This study was conducted with the aim of determining the factors that affect the adoption of accounting information system software. In this framework, an accounting information system is a system that a business uses for collecting, storing, managing, and reporting its financial data. To test the hypothesis that there is no significant difference in the factors affecting the adoption of accounting information system software as determined by the managers and its effects on the performance of the small and medium enterprises at Santa Cruz, Laguna, chi-square of independence was used. The data were collected using Google Forms. A total of eighty (80) respondents participated in the survey. The results show that only two factors, namely organizational and technological factors, have significantly affected the adoption of accounting information system software, and using AIS software can reduce the operational costs of small and medium enterprises. The findings suggest that management must be fully committed to adopting AIS to have effective decision-making, fasten the accounting or business transactions, and improve organizational performance.